图片
荣富国际logo
会员登录
登录账号:
登录密码:
验 证 码:
您好,您已登录
您有条新到站内短信
会员中心 退出登录
图片
点评分类
点评搜索
点评详情
发布于:2019-1-8 11:08:40  访问:50 次 回复:0 篇
版主管理 | 推荐 | 删除 | 删除并扣分
Eously observed that a rise in cell migration was accompanied with
Urolithin A Additionally, JNK1/2 mediated PGE2-induced VidofludimusPurity & Documentation expression of uPA and MMP-9 in LoVo cells. Additionally, JNK1/2 mediated PGE2-induced expression of uPA and MMP-9 in LoVo cells. (3) On the other hand, PGE2 (ten -6 M) treatment showed no influences on regulating the expression of PAI-1, TIMP-1, TIMP-2, TIMP-3 and TIMP-4 in LoVo cells. (four) PGE2-induced expression of uPA and MMP-9 in human LoVo cells was substantially inhibited by 17bestradiol (10 -8 M) pretreatment. 17b-Estradiol significantly inhibited PGE2-induced uPA and MMP-Hsu et al. Journal of Biomedical Science 2011, 18:61 http://www.jbiomedsci.com/content/18/1/Page six ofFigure three PGE2 upregulates uPA and MMP-9 through JNK1/2 signaling pathway in human LoVo colon cancer cells. (A) LoVo cells had been pretreated with automobile, LY294002 (Akt activation inhibitor), U0126 (ERK1/2 activation inhibitor, 1 M), SB203580 (p38 MAPK inhibitor, 1 M), SP600125 (JNK1/2 inhibitor, 1 M) or QNZ (NFB inhibitor, 1 M) for 1 h and followed by PGE2 (10-6M) administration for 24 h, and after that were harvested for immunoblotting assays. (B) LoVo cells cultured in DMEM have been treated with PGE2 (10-6M) for different periods (15 min, 30 min, 1 h, three h, six h, 12 h and 24 h), and subsequently measured the phosphorylation/activation of proteins by immunoblotting assay. The fold ratio of pJNK1/2 and JNK1/2 was measured. Total protein of cell extracts was separated by 12 SDS-PAGE, transferred to PVDF membranes, and immunoblotted with antibodies against uPA, MMP-9 (A), phospho-JNK1/2 and JNK1/2 (B) PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/26577270 proteins. Equal loading was assessed with an anti-atubulin antibody. **, p < 0.01 versus control (mean ?SE, n = 3).Hsu et al. Journal of Biomedical Science 2011, 18:61 http://www.jbiomedsci.com/content/18/1/Page 7 ofFigure 4 17b-Estradiol down-regulates PGE2-induced uPA and MMP-9 expression by suppressing activation of JNK1/2 in human LoVo cells. (A) LoVo cells cultured in DMEM were treated with 17b-estradiol (10-8M) for various periods (5 min, 15 min, 30 min, 1 h, 3 h, 6 h, 12 h and 24 h), and subsequently measured the phosphorylation/activation of proteins by immunoblotting assay. The fold ratio of p-JNK1/2 and JNK1/2 was measured. (B) LoVo cells were pretreated with 17b-estradiol (10-8M) for 30 min, followed by PGE2 (10-6M) treatment for 30 min or 24 h, and then were subjected to immunoblotting assay for protein detection of phospho-JNK1/2 (PGE2 stimulation within 30 min); uPA and MMP9 (PGE2 stimulation within 24 h).expression by suppressing activation of JNK1/2. PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/26437915 These final results demonstrate that 17b-estradiol may effectively inhibit PGE2-induced motility in human LoVo colon cancer cells (Figure six). Upregulation of MMPs is reported to contribute to ECM remodeling, tumor cell invasion and metastasis, hence leading to the development of malignant tumor [5]. Each mRNA levels MMP-2 and MMP-9 happen to be found to be overexpressed in colon carcinomas [21,22]. Within the observations of Collins et al.
共0篇回复 每页10篇 页次:1/1
共0篇回复 每页10篇 页次:1/1
我要回复
回复内容
验 证 码
看不清?更换一张
匿名发表 
图片
脚注信息
版权所有 Copyright(C)2009-2020  荣富国际茶叶贸易有限公司